RapidPath: Single-Step, Rapid Detection of Bacteria in Environmental Waters," aims to significantly advance the field of environmental monitoring through the development of cutting-edge biosensor technology. This innovative system is designed for the rapid, accurate, and on-site detection of microbial contaminants in environmental water sources, a capability that is increasingly critical in our efforts to ensure water safety and protect public health. At the heart of RapidPath assay lies the integration of Innovative oligo constructs, a novel approach that combines the high specificity and signal amplification for target microbial DNA to produce a quantifiable signal indicative of the presence of pathogens. This biosensor is capable of delivering results within 20 minutes, drastically reducing the time required for water quality analysis compared to traditional laboratory-based methods. What sets RapidPath apart is its single-step, field-operable functionality. Designed to be user-friendly, it does not require specialized laboratory equipment or extensive technical training, making it accessible to a broad range of users from field technicians to environmental scientists. This feature is particularly beneficial for remote or resource-limited settings where access to laboratory facilities is challenging. The development of RapidPath is timely and aligns with the objectives outlined in the Environmental Act 2021, which emphasizes the importance of continuous and real-time monitoring of watercourses to prevent harmful discharges and protect biodiversity. By providing a tool for the immediate assessment of biological contamination, RapidPath supports compliance with regulatory standards and enhances environmental stewardship. As we move towards the project's implementation, we are committed to ensuring that RapidPath represents a sustainable and cost-effective solution for water quality monitoring. The technology's adaptability for future integration into fixed installation kiosks with automated data collection and analysis capabilities further underscores its potential to transform water management practices. Our vision for RapidPath is not merely to introduce a new product but to set new standards in water quality assessment, contributing to the global effort to secure clean water for all. We believe that by enhancing our ability to monitor and respond to environmental challenges effectively, we can make significant strides towards achieving net-zero emissions and fostering a healthier, more sustainable world.

CEXAL LTD is delighted to present our innovative project focused on developing a cutting-edge cell-free biosensing system called the Dual-Cas9-Oligo (CasDuO) for the rapid detection of microorganisms in water samples. This pioneering project leverages engineering biology principles to overcome the limitations of traditional microbiological testing methods. By harnessing genetic circuitry, our biosensors will generate measurable signals upon detecting target microorganisms, enabling quick and accurate on-site monitoring. **Improved Water Testing:** CEXAL's cell-free CasDuO biosensing system will offer a reliable and rapid alternative to traditional microbiological testing methods. This breakthrough technology will greatly expedite water quality assessment and ensure timely response to potential contaminants. **Cost-effectiveness**: CEXAL's cell-free CasDuO biosensing system will offer a reliable and rapid alternative to traditional microbiological testing methods. This breakthrough technology will greatly expedite water quality assessment and ensure timely response to potential contaminants. Through vertical integration and reduced reliance on suppliers, we expect to control manufacturing costs effectively. This cost efficiency will translate into more affordable biosensing products, making them accessible to a broader range of users. **Environmental Benefits**: With the ability to detect contaminants at lower concentrations, our CasDuO biosensors will enable earlier identification of potential environmental risks. Timely interventions can help protect ecosystems and public health.

This project aims to test novel engineered biological detection mechanisms to detect specific pathogenic microbial strains in low volumes of liquids. The project is to be carried out from 1 June 2023 until 30 November 2023\. The project has been devised with the view of developing and manufacturing a rapid test kit or other molecular testing solutions to detect pathogens in natural waters, recreational waters and distributed and treated water supplies.